TitleHuman haematopoietic stem cell lineage commitment is a continuous process
Data descriptionSingle-cell RNA-seq of 1,034 (for I1) and 379 (for I2) human haematopoietic stem cells (HSCs)
Doi10.1038/ncb3493(Mitchell et al., 2016)
Web-link of the paperhttps://www.nature.com/articles/ncb3493
Data typescRNA-seq
DatabaseGene Expression Omnibus (GEO)
Accession numberGSE75478
URL of the datahttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE75478
Tissuebone marrow
Cell typehaematopoietic stem cells (HSCs)
Number of cells1,034 (for I1) and 379 cells (for I2)
Cell capture platformFlow cytometry
Library preparation protocolQuartz-Seq, SMART-seq2
Unique molecular identifier (Y/N)N
Spike-in (Y/N)Y
Full-length (Y/N)N
Brief summary of the scientific questionThe authors integrated flow cytometric, transcriptomic and functional data at single-cell resolution to quantitatively map early differentiation of human HSCs towards lineage commitment.
Brief summary of the bioinformatics processingReads were aligned to the Homo sapiens genome using GSNAP and counted using HTSeq. Data normalization using posterior odds ratio. Used Ward linkage on Euclidean distances. Gap statistics was computed on hierarchical clustering function using the R package cluster.
CitationVelten L, Haas SF, Raffel S, Blaszkiewicz S, Islam S, Hennig BP, Hirche C, Lutz C, Buss EC, Nowak D, Boch T, Hofmann WK, Ho AD, Huber W, Trumpp A, Essers MA, Steinmetz LM. Human haematopoietic stem cell lineage commitment is a continuous process [J]. Nat Cell Biol. 2017, 19(4):271-281
Web-link of the paperhttps://www.nature.com/articles/ncb3493